Pink bands can be visualized with a 532 nm laser or green LED. Fluorescent detection - blue bands can be visualized with a 635 nm laser or red LED.Strep-tag affinity peptide for detection and MW determination on western blots.Prestained bands for monitoring of electrophoresis progression and assessment of blotting efficiency.Dual-color prestained bands (reference bands prestained pink, all others prestained blue).Proprietary staining technology that provides batch-to-batch MW consistency and reproducible electrophoretic migration Western blotting protein standards can be used for both fluorescent visualization and colormetric or chemiluminescent immunodetection on western blots.Highly accurate MW estimation, r 2 values >0.99.Clean, sharp bands with MW confirmed by mass spectrometry.Features and Benefits of WesternC Western Blotting Protein Standards These protein standards contain ten highly purified recombinant proteins with molecular masses from 10 to 250 kD and include three high-intensity reference bands (25, 50, and 75 kD). WesternC Standards for protein electrophoresis and blotting offer unsurpassed band sharpness, accurate MW determination, lot-to-lot consistency, and excellent blotting results. Precision Plus Protein™ WesternC™ Protein Standards are prestained, broad-range protein standards that enable chemiluminescent detection when probed with StrepTactin-HRP conjugates, so the protein standard appears directly on a film or CCD image. Read their story and request a free set of matching (temporary) tattoos here. (B) lysate sonication is critical to the observation of nuclear and chromatin-bound proteins. It is useful to include both types of markers for both monitoring experiment progression and ease of interpreting results. The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition.Two students celebrated their love of science with Precision Plus Protein Kaleidoscope Ladder tattoos. The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. Using a biotinylated marker offers visualization of the ladder along with antibody targets on film. Consisting of recombinant proteins fused to an IgG binding site, these versatile molecular weight ladders bind the primary or secondary antibody used for the detection of the target protein. Tested in polyacrylamide gel electrophoresis and Western Blotting. Western blot protein ladders are designed for protein molecular weight estimation directly on the blot during detection. Detectable using a fluorescence-based imager (laser scanner) or CCD-based imager. One prominent feature of this protein ladder, is that it remains in the liquid state at the storage temperature therefore, there is not a freeze-thaw cycle involved, making the ladder very stable.Ġ.1~0.4 mg/ml of each protein in 20 mM Trisphosphate (pH 7.5 at 25☌), 2% SDS, 1 mM reducing reagent, 3.6 M Urea, and stabilizer.įor each application, 5-15µl is needed, depending on the size of the wells. Fluorescent Rainbow markers (Mr 12 000 to 225 000) Visible Rainbow marker to monitor migration and transfer (for gels and blots, respectively). No need to heat, dilute, or add reducing agent before loading. The ladder is supplied in protein gel loading buffer and is ready-to-use. It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for sizing of proteins. The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. Use 5-15µl, depending on the size of the wellsĪpplication: The Bullseye Pre-stained Protein Ladder is suitable for visualizing proteins during electrophoresis without staining and for monitoring transfer onto membrane.2 reference bands - red at ~75kDa and green at ~25kDa.Stoolfix Gut Microbiome Stabilization Kitsīullseye Pre-stained Protein Ladder, 10-180 kDa Visualization of protein migration during SDS-polyacrylamide gel electrophoresis Verification of protein transfer onto membranes after Western blotting. 5 l of Prestained Protein Ladder V resolves 10 bands in 4-20 SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane. mPAGE ® Western Protein Standards consist of seven recombinant proteins at 20 kDa, 30 kDa, 40 kDa, 50 kDa, 60 kDa, 80 kDa and 120 kDa with an IgG binding site that binds to most antibodies.Beakers, Graduated Cylinders, and Flasks.Find help and support for your protein electrophoresis, western blotting and western blot detection questions including useful resources, tips and troubleshooting support related to our protein stains, standards and ladders. to visualize protein ladders on Western blots is to use prestained protein ladders which remain visible when transferred to the blotted membrane. 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